Takara SMARTer Human TCR a/b Profiling Kit

Obtain full-length sequences of TCR-alpha and TCR-beta V(D)J variable regions

NOTE:
For TCR sequencing, we recommend the upgraded version, the SMARTer Human TCR a/b Profiling Kit v2 (TCR v2).
Based on the same great chemistry, TCR v2 improves upon it with:

• UMIs for confident clonotype calls
• UDIs for increased multiplexing capabilities
• Easy-to-use bioinformatics software for an end-to-end solution

Cat. #635014 (12 rxns) and Cat. #635015 (48 rxns) are no longer available to order.

Product Overview

The SMARTer Human TCR a/b Profiling Kit provides a powerful solution for T-cell receptor (TCR) repertoire analysis using next-generation sequencing (NGS).
The kit employs a 5′ RACE-like approach to capture complete V(D)J variable regions of TCR transcripts, starting from:

• Human blood RNA samples (10 ng–3 µg), or
• Intact lymphocytes (50–10,000 purified T cells)

It enables generation of data for both TCR-alpha and TCR-beta chain diversity, either in the same experiment or separately.
Unlike multiplex PCR-based methods, each round of library amplification uses a single primer pair per TCR subunit.
Libraries are indexed and ready for sequencing on Illumina platforms.

This kit leverages proven SMART technology (Switching Mechanism at 5′ End of RNA Template) and LNA technology to amplify TCR mRNA sequences in an unbiased manner with high sensitivity.

Key Features

• Complete V(D)J sequence information — Obtain full-length variable region sequences of TCR mRNA transcripts
• TCR-alpha and TCR-beta profiling — Analyze diversity for both subunits in the same or separate experiments
• Flexible sample inputs — Start from 10 ng–3 µg RNA or 50–10,000 purified T cells
• No multiplex PCR required — Single primer pair per subunit ensures unbiased amplification
• Illumina-ready libraries — Incorporates adapters and indexes without ligation; multiplex up to 96 libraries
• Exceptional sensitivity — Detect low-abundance TCR sequences even with shallow read depth
• Excellent reproducibility — Consistent results across varying input amounts

Applications

• Human TCR repertoire analysis (TCR-alpha and TCR-beta subunits)

Additional Product Information

Refer to the product’s Certificate of Analysis for details on:

• Storage conditions
• Product components
• Technical specifications

See the Kit Components List for detailed component information.
Certificates of Analysis and Kit Components Lists are available under the Documents tab.