Thermo Fisher Scientific Triadin Monoclonal Antibody (IIG12)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Published species

Mouse, Rabbit

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

IIG12

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2256496

Product Specific Information

MA3-931 detects triadin from mouse and rabbit skeletal muscle tissues. This antibody does not detect triadin from cardiac tissues.

MA3-931 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects a single ~95 kDa band representing triadin in rabbit skeletal muscle extracts. Immunofluorescence staining of triadin in rabbit skeletal muscle with MA3-931 results in its co-localization with ryanodine receptor, which is consistent with the association of ryanodine receptor and triadin in triads.

The MA3-931 antigen is rabbit skeletal muscle triads.

Target Information

The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPRs) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyRs), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, triadin (MW 95 kDa) transmits electrochemical signals to the SR through direct interaction with both DHPRs and RyRs. Though its exact role in this signaling process is unclear, triadin has been shown to co-localize with both DHPR and RYR at the junctional face of the terminal cisternae.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.