Thermo Fisher Scientific ATP1A1 Monoclonal Antibody (M8-P1-A3)
ATP1A1 단백질을 인식하는 Thermo Fisher Scientific의 M8-P1-A3 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry 등에 사용 가능. 다양한 종에 반응하며 고순도 Protein A 정제. 연구용으로 안정적이며 높은 특이성을 제공.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:200–1:2,000 | 35 publications |
| Immunohistochemistry (IHC) | – | 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:2,000 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100 | – |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | 7 publications |
| Flow Cytometry (Flow) | 1:20–1:100 | – |
| Neutralization (Neu) | – | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Sheep, Pig, Rat |
| Published Species | Bovine, Dog, Human, Invertebrate, Mouse, Non-human primate, Pig, Rat, Sheep, Yeast |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M8-P1-A3 |
| Immunogen | Lamb kidney alpha 1 sodium/potassium ATPase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2061127 |
Product Specific Information
MA3-929 detects sodium/potassium ATPase alpha 1 in sheep, canine, rat, mouse, human, and porcine tissues.
This antibody does not detect the alpha 1 isoform in Xenopus and chicken, nor the alpha 2 or alpha 3 isoform in rat.
It has been successfully used in Western blot, immunofluorescence, immunocytochemistry, flow cytometry, and immunohistochemistry procedures.
By Western blot, this antibody detects an ~110 kDa protein representing alpha 1 sodium/potassium ATPase from canine kidney extract.
Immunohistochemical staining yields a pattern consistent with plasma membrane localization.
The antigen is sheep kidney alpha 1 sodium/potassium ATPase, recognizing an epitope between amino acid residues 496–506 of lamb kidney sodium/potassium ATPase.
Target Information
The protein encoded by this gene belongs to the family of P-type cation transport ATPases and to the subfamily of Na+/K+-ATPases.
Na+/K+-ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane.
These gradients are essential for osmoregulation, sodium-coupled transport of various molecules, and electrical excitability of nerve and muscle cells.
The enzyme consists of two subunits: a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta).
This gene encodes the alpha 1 subunit, with multiple transcript variants encoding different isoforms identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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