Thermo Fisher Scientific IL-10 Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 Rabbit Polyclonal Anti-Mouse IL-10 항체로, Western blot과 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS 내 동결건조 형태로 제공됩니다. 면역조절 연구 및 사이토카인 분석용으로 이상적입니다.
- 카탈로그번호
- 500-P60BT-500UG
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-10 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | Detection limit: 1.5–3.0 ng/lane under reducing or non-reducing conditions |
| ELISA | 0.25–1.0 µg/mL | Detects 0.2–0.4 ng/well recombinant mIL-10 in sandwich ELISA |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived Recombinant Murine IL-10 (amino acids 19–178) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| Catalog Size | 500-P60BT-1MG (2 × 500 µg) |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGQKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLEDQGVYKA MNEFDIFINC IEAYMMIKMK S
Preparation:
Produced from sera of rabbits immunized with highly pure recombinant murine IL-10. The anti-murine IL-10-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
For detection of mIL-10, use 0.25–1.0 µg/mL of this antibody in conjunction with PeproTech Polyclonal Anti-Murine IL-10 (500-P60) as a capture antibody. Detects at least 0.2–0.4 ng/well of recombinant mIL-10.
Western Blot:
For detection of mIL-10, use 0.1–0.2 µg/mL of this antibody. Detection limit for recombinant mIL-10 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
IL-10 encodes a cytokine primarily produced by monocytes, with additional production by lymphocytes. It regulates immune responses and inflammation by down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. IL-10 enhances B cell survival, proliferation, and antibody production, inhibits NF-kappa B activity, and participates in the JAK-STAT signaling pathway. Knockout studies indicate IL-10 is essential for intestinal immune regulation. Mutations in IL10 are linked to increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Associated Diseases:
Graft-Versus-Host Disease, Human Immunodeficiency Virus Type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.