Thermo Fisher Scientific IL-10 Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 Rabbit Polyclonal Anti-Mouse IL-10 항체로, Western blot과 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS 내 동결건조 형태로 제공됩니다. 면역조절 연구 및 사이토카인 분석용으로 이상적입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | Detection limit: 1.5–3.0 ng/lane under reducing or non-reducing conditions |
| ELISA | 0.25–1.0 µg/mL | Detects 0.2–0.4 ng/well recombinant mIL-10 in sandwich ELISA |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived Recombinant Murine IL-10 (amino acids 19–178) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| Catalog Size | 500-P60BT-1MG (2 × 500 µg) |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGQKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLEDQGVYKA MNEFDIFINC IEAYMMIKMK S
Preparation:
Produced from sera of rabbits immunized with highly pure recombinant murine IL-10. The anti-murine IL-10-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
For detection of mIL-10, use 0.25–1.0 µg/mL of this antibody in conjunction with PeproTech Polyclonal Anti-Murine IL-10 (500-P60) as a capture antibody. Detects at least 0.2–0.4 ng/well of recombinant mIL-10.
Western Blot:
For detection of mIL-10, use 0.1–0.2 µg/mL of this antibody. Detection limit for recombinant mIL-10 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
IL-10 encodes a cytokine primarily produced by monocytes, with additional production by lymphocytes. It regulates immune responses and inflammation by down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. IL-10 enhances B cell survival, proliferation, and antibody production, inhibits NF-kappa B activity, and participates in the JAK-STAT signaling pathway. Knockout studies indicate IL-10 is essential for intestinal immune regulation. Mutations in IL10 are linked to increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Associated Diseases:
Graft-Versus-Host Disease, Human Immunodeficiency Virus Type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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