Thermo Fisher Scientific IL-9 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MQRCSTTWGI RDTNYLIENL KDDPPSKCSC SGNVTSCLCL SVPTDDCTTP CYREGLLQLT NATQKSRLLP VFHRVKRIVE VLKNITCPSF SCEKPCNQTM AGNTMSFLKS LLGTFQKTEM QRQKSRP.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-9. Anti-Murine IL-9-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Murine IL-9 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-9 (500-P59) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Murine IL-9.

Western Blot:
To detect Murine IL-9 by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Murine IL-9 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P59BT-1MG will be provided as 2 × 500 µg.

Target Information

Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated. TGF beta plays an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving differentiation of Th9 cells directly.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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