Thermo Fisher Scientific LDHA Polyclonal Antibody
LDHA 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC/IF, Flow Cytometry에 적합합니다. Human, Mouse, Rat 반응성이 있으며, 항원 친화 크로마토그래피로 정제되었습니다. 동결건조 형태로 제공되며, 재구성 시 500 µg/mL 농도입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived human LDHA recombinant protein (Position: A2–R106) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5 mg BSA |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2746708 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Lactate dehydrogenase (LDH) is an enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD⁺.
In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form).
The three human LDHs have 84–89% sequence similarities and 69–75% amino acid identities.
There are five LDH isoenzymes based on the proportion of M and H chains in the tetrameric structure.
LDHA is hypoxia-inducible and regulated by HIF1α, while LDHB is often upregulated in cancers.
Mutations in the LDHA gene can cause Glycogen Storage Disease 11 (GSD11).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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