Thermo Fisher Scientific LIF Polyclonal Antibody
Thermo Fisher Scientific의 LIF Polyclonal Antibody는 인간 LIF 단백질을 인식하는 토끼 IgG 형 다클론 항체입니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 동결건조 형태로 제공되며 재구성 후 500 µg/mL 농도로 사용 가능합니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived human LIF recombinant protein (Position: S23–F202) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5 mg BSA |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2746715 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Leukemia inhibitory factor (LIF) is a 20 kDa protein belonging to the IL-6 receptor family. It binds to a heterodimeric membrane receptor composed of a LIF-specific subunit (gp190 or LIFR) and the shared subunit gp130. LIF is expressed in various tissues including thymus, lung, and neuronal tissue, and can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17. Elevated LIF levels have been observed in rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. It is best known for inhibiting differentiation of embryonic stem cells in mice, contributing to stem cell self-renewal. Human and mouse LIF share 79% sequence homology and exhibit cross-species activity, though stem cell differentiation inhibition appears mouse-specific.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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