
Thermo Fisher Scientific BUB1B Monoclonal Antibody (OTI6E5), TrueMAB
인간 BUB1B 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC, ICC/IF, Flow Cytometry에 사용 가능. 동결건조 형태로 제공되며, 고순도 친화 크로마토그래피 정제. 세포 분열 체크포인트 연구 및 암 관련 단백질 분석에 적합.
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Thermo Fisher Scientific BUB1B Monoclonal Antibody (OTI6E5), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6E5 |
| Immunogen | Full-length protein expressed in 293T cells transfected with human BUB1B expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve approximately 1 mg/mL antibody concentration.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
BUBR1 is a Ser/Thr protein kinase involved in the mitotic checkpoint, ensuring proper chromosome attachment before anaphase. Defects in spindle checkpoint function can lead to genetic instability and cancer progression.
Human cells express two related kinases, hBUBR1 and hBUB1, which monitor kinetochore-microtubule interactions. BUBR1 is essential for normal mitosis and has been implicated in various human cancers, including breast cancer, through its interaction with BCSG1.
Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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