Thermo Fisher Scientific Calnexin Monoclonal Antibody (CANX, 1541)
Calnexin 단백질을 인식하는 Mouse Monoclonal Antibody로, WB, IHC(P), ICC/IF에 사용 가능. Human 시료에 반응하며 Rat에는 반응하지 않음. Protein A/G로 정제된 1 mg/mL 액상 제품으로, PBS(pH 7.4)에 보존제 없이 제공됨.
- 카탈로그번호
- 821-MSM1-P1ABX
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL | |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CANX, 1541 |
| Immunogen | Recombinant N-terminal fragment of human Calnexin protein (around aa 1–300) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | –20°C or –80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Tested in direct ELISA (use antibody at 2–4 µg/mL for coating).
- Does not react with Rat.
- For Immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues: heat sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes.
Target Information
Calnexin (also known as IP90, p88, p90) is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). It functions as a molecular chaperone, assisting in proper folding and assembly of polypeptides into multisubunit proteins.
Calnexin associates with MHC class I heavy chains, partial T cell receptor complexes, and B cell membrane immunoglobulin, but not with completed receptor complexes. It retains incompletely folded proteins in the ER.
Unlike soluble ER resident proteins that contain the KDEL sequence, calnexin lacks KDEL but has positively charged cytosolic residues for ER retention.
Calnexin structure includes:
- ER luminal domain: 461 amino acids
- Transmembrane segment: 22 amino acids
- Cytoplasmic tail: 89 amino acids
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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