Thermo Fisher Scientific Apolipoprotein J Recombinant Rabbit Monoclonal Antibody (SAIC-43B-8)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.03 µg/mL

-

Immunocytochemistry (ICC/IF)

1:100

-

Flow Cytometry (Flow)

1:100

-

ELISA (ELISA)

Assay-dependent

-

Mass Spectrometry (MS)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SAIC-43B-8

Immunogen

Peptide ASSIIDELFQDR derived from Clusterin conjugated to KLH. Clusterin isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Apolipoprotein J,uniProtId:P10909-1,ncbiNodeId:9606,antigenRange:183-194,antigenLength:449,antigenImageFileName:MA5-47923_Apolipoprotein_J_P10909-1_Rabbit.svg,antigenImageFileNamePDP:MA5-47923_Apolipoprotein_J_P10909-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074367

Product Specific Information

Specificity: Recognises human CLU.

Target Information

Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.