Thermo Fisher Scientific Phospho-PP2A alpha (Tyr307) Recombinant Rabbit Monoclonal Antibody (ST49-05)
Phospho-PP2A alpha (Tyr307) 단백질을 검출하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체. Western blot, IHC, ICC/IF에 적합하며 높은 특이성과 민감도를 제공. 로트 간 일관성 및 동물 유래 성분 없는 포뮬레이션.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ST49-05 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Tyr307 of Human PP2A (aa 260–309) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809447 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences are cloned from immunoreactive rabbits, and individual clones are screened to select optimal candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Protein phosphorylation and dephosphorylation on serine and threonine residues regulate many cellular processes such as division, homeostasis, and apoptosis. Protein phosphatases, including PP1, PP2A, PP2B (calcineurin), and PP2C, are key enzymes in these pathways.
PP2A exists as a trimeric complex composed of regulatory, variable, and catalytic subunits, with subfamily members PP2A alpha and PP2A beta. Another related phosphatase, PPX (also known as PP4), is considered part of a novel PP family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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