Thermo Fisher Scientific RAB5 Recombinant Rabbit Monoclonal Antibody (ST47-04)
RAB5 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC 등 다양한 응용에 적합합니다. 높은 특이성과 재현성을 제공하며, Protein A 정제 및 동물 유래 성분이 없는 포뮬레이션입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ST47-04 |
| Immunogen | Synthetic peptide within Human Rab5 (aa 166–215) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809440 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
RAB5 belongs to the small GTPase Rab family, key regulators of intracellular membrane trafficking from vesicle formation to membrane fusion.
RAB5A is required for fusion of plasma membranes and early endosomes, contributes to filopodia extension, and regulates the exosomal release of SDCBP, CD63, PDCD6IP, and syndecan. It also plays a role in maturation of apoptotic cell-containing phagosomes, likely downstream of DYN2 and PIK3C3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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