Thermo Fisher Scientific Phospho-JNK1/JNK2/JNK3 (Thr183, Thr221) Recombinant Rabbit Monoclonal Antibody (ST500)
인간, 마우스, 랫트 시료에 반응하는 재조합 래빗 모노클로날 항체로 JNK1/2/3의 인산화 형태(Thr183, Thr221)를 검출. Western blot, IHC, ICC/IF, Flow cytometry에 적합. 높은 특이성과 민감도, 로트 간 일관성 및 동물 유래 성분 없음.
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Applications and Tested Dilutions
| Application | Tested Dilution Range |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ST500 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Thr183 and Thr221 of Human JNK1/2/3 (aa 161–204) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809448 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to large rabbit immune repertoire
Target Information
C-Jun N-terminal Kinase (JNK), also known as Mitogen-activated protein kinase (MAPK8), is part of the MAPK superfamily of stress-activated protein kinases. MAPKs are serine-threonine kinases activated by extracellular stimuli, mediating signal transduction from the cell surface to the nucleus.
Functions and Biological Roles:
- Regulates proliferation, differentiation, transcription, and development
- Activated by stress and inflammatory signals
- Expressed as ten isoforms via mRNA splicing, predominantly JNK1 and JNK2
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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