Thermo Fisher Scientific CXCL10 Polyclonal Antibody, PeproTech
CXCL10(IP-10)에 특이적인 Rabbit Polyclonal Antibody로, Western blot과 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, Human 및 Mouse 반응성이 있습니다. 연구용으로만 사용되며, -20°C에서 보관합니다.
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Applications 및 권장 희석 배수
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | View 3 publications |
| Neutralization (Neu) | – | View 1 publication |
| In vitro Assay (IV) | – | View 1 publication |
| Immunostaining (IS) | – | View 10 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IP-10 (CXCL10) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930863 |
Product Specific Information
AA Sequence of Recombinant Protein:
VPLSRTVRCT CISISNQPVN PRSLEKLEII PASQFCPRVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK ERSKRSP.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IP-10 (CXCL10). Anti-Human IP-10 (CXCL10)-specific antibody was purified by affinity chromatography employing an immobilized Human IP-10 (CXCL10) matrix.
Sandwich ELISA:
To detect Human IP-10 (CXCL10) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. In conjunction with PeproTech Biotinylated Anti-Human IP-10 (CXCL10) (500-P93Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human IP-10 (CXCL10).
Western Blot:
To detect hIP-10 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hIP-10 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
IP-10 (interferon-gamma induced protein 10 kD; in mouse also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene belonging to the CXC chemokine superfamily. It was first cloned in 1985.
The biological functions of IP-10 include:
- Stimulation of monocytes, natural killer, and T-cell migration
- Regulation of T-cell and bone marrow progenitor maturation
- Modulation of adhesion molecule expression
- Inhibition of angiogenesis
Mig and IP-10 share the same receptor, CXCR3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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