Thermo Fisher Scientific MIP-3 alpha Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived, 8.0 kDa Recombinant Human MIP-3alpha (CCL20) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CCL20,uniProtId:P78556-1,ncbiNodeId:9606,antigenRange:27-96,antigenLength:96,antigenImageFileName:500-P95ABT-25UG_CCL20_P78556-1_Rabbit.svg,antigenImageFileNamePDP:500-P95ABT-25UG_CCL20_P78556-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930879

Product Specific Information

AA Sequence of recombinant protein: ASNFDCCLGY TDRILHPKFI VGFTRQLANE GCDINAIIFH TKKKLSVCAN PKQTWVKYIV RLLSKKVKNM.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-3alpha (CCL20). Anti-Human MIP-3alpha (CCL20)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human MIP-3alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MIP-3alpha (500-P95A) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-3alpha.

Western Blot: To detect Human MIP-3alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human MIP-3alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P95ABT-1MG will be provided as 2 x 500 µg

Target Information

Macrophage Inflammatory Protein-3 alpha (MIP-3alpha), also called CCL20, is expressed in the liver, lungs, lymph nodes and peripheral blood lymphocytes. MIP-3alpha is strongly up regulated by inflammatory signals, and down regulated by the anti-inflammatory cytokine IL-10. MIP3alpha signals through the G protein-coupled receptor, CCR6, and acts as a chemoattractant to lymphocytes and dendritic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.