Thermo Fisher Scientific Human Caspase 8 Synthetic Peptide

Applications

Control (Ctrl)

• Assay-dependent

Blocking Assay (BLOCK)

• Assay-dependent

Product Specifications

Product Specific Information

This peptide corresponds to 16 amino acids near the carboxy terminus of human Caspase-8 isoform A.
PEP-0236 can be used as a blocking peptide with polyclonal antibody PA5-20118.

Target Information

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a pro-domain, a large protease subunit, and a small protease subunit. Activation requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits.

Caspase 8 is involved in programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain suggests interaction with Fas-interacting protein FADD. Caspase 8 was detected in the insoluble fraction of affected brain regions from Huntington disease patients but not in normal controls, suggesting a role in neurodegenerative diseases.

Activated Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DED domain present in tandem in the pro-form of Caspase 8. It subsequently activates downstream caspases including Caspase 9, committing the cell to apoptosis. Caspase 8 also reacts with Jurkat cells and Tonsil. Overexpression induces apoptosis, which can be blocked by ICE family-specific inhibitors. Multiple alternatively spliced transcript variants encoding different isoforms have been described, though not all have complete sequence information.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.