Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Alexa Fluor 532, eBioscience

Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Alexa Fluor 532, eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with CD3 gamma and delta, the epsilon chain is essential for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Crosslinking via immobilized UCHT1 initiates intracellular biochemical pathways leading to activation and proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and validated for flow cytometry of human peripheral blood cells
  • Recommended usage: 5 µL (0.25 µg) per test
  • Test volume: 100 µL
  • Cell number: 10⁵–10⁸ cells/test (empirical determination required)

Fluorochrome Information:
Alexa Fluor® 532 is excited with a 532 nm green laser and emits at 561 nm. Not compatible with the 561 nm yellow-green laser. Recommended filter: 560/14 band pass. Ensure instrument compatibility.

Excitation: 532 nm
Emission: 561 nm
Laser: Green Laser

Target Information

The CD3 complex is crucial for transducing antigen-recognition signals into T cells and regulating TCR surface expression. It includes CD3 gamma, delta, epsilon, and zeta subunits, encoded by genes on human chromosome 11. These subunits have long cytoplasmic tails that associate with signal transduction molecules via a double tyrosine-based motif.

CD3 is expressed on:

• 68–82% of normal peripheral blood lymphocytes
• 65–85% of thymocytes
• Purkinje cells in the cerebellum

It is not expressed on B or NK cells. Decreased T lymphocyte percentages may occur in autoimmune diseases. Defects in CD3 genes are associated with CD3 immunodeficiency.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

Available Formats

Unconjugated, Alexa Fluor 561, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV/Violet/Yellow series, eFluor, FITC, PE series, Super Bright series, NovaFluor series, and custom conjugation available.

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