Thermo Fisher Scientific KDEL Monoclonal Antibody (2D6), APC
KDEL 서열을 인식하는 단클론 항체로, APC 형광단과 결합되어 ER 관련 단백질 검출에 적합. Western blot에서 1:1,000 희석으로 사용 가능. Human, Mouse, Rat 시료 반응. 단백질 G 정제, 보존제 없음, 4°C 보관.
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Applications
- Western Blot (WB): Tested at 1:1,000 dilution
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D6 |
| Immunogen | KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932327 |
Additional Formats
- Unconjugated (MA5-27581)
- FITC (MA5-45874)
- PE (MA5-45876)
- PerCP (MA5-45875)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45873 was sufficient for detection of KDEL in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects KDEL-tagged proteins.
Target Information
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. GRP78 (78 kDa) and GRP94 (94 kDa) as well as protein disulfide isomerase (PDI) share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL is necessary for ER retention and sufficient to reduce secretion of proteins from the ER. This retention is mediated by a KDEL receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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