Thermo Fisher Scientific ARAF Monoclonal Antibody (OTI2F8), TrueMAB
인간 ARAF 단백질(aa 145–365)을 인식하는 mouse IgG1 단클론 항체로, WB 및 IHC(P) 실험에 적합. 무보존제, 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용 가능. 신호전달 관련 Raf-1 및 A-Raf 연구용에 최적.
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Thermo Fisher Scientific ARAF Monoclonal Antibody (OTI2F8), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 145–365 of human ARAF produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
Several serine/threonine protein kinases act as intermediates in signal transduction pathways, including ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1.
Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity, expressed broadly across cell lines and homologous to v-Raf, a viral transforming gene product.
A-Raf, another member of the Raf family, shares significant homology with Raf-1 within the kinase domain but less elsewhere.
A-Raf expression is highest in urogenital tissues and kidney and lowest in brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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