Thermo Fisher Scientific ARAF Monoclonal Antibody (OTI2G4), TrueMAB
ARAF 단백질을 인식하는 마우스 단클론 항체로, Western blot에 적합합니다. Human ARAF(aa 145-365) 재조합 단백질로부터 제작되었으며, 무보존제 PBS 버퍼에 동결건조 형태로 제공됩니다. 연구용으로 신호전달 관련 단백질 분석에 유용합니다.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2G4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 145–365 of human ARAF produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, it is recommended to perform an additional desalting step (e.g., using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Several serine/threonine protein kinases act as intermediates in signal transduction pathways, including ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1.
Raf-1 is a cytoplasmic protein with intrinsic serine/threonine kinase activity and is the cellular homolog of v-Raf, the transforming gene product of the 3611 murine sarcoma virus.
A-Raf, another member of the Raf gene family, shares substantial homology with Raf-1 within the kinase domain but less in other regions.
A-Raf expression is highest in urogenital tissues and kidney, and lowest in brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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