Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (9E2), NovaFluor Blue 610-70S, eBioscience

Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (9E2), NovaFluor Blue 610-70S

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.8 µg)/test
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for normal human peripheral blood cells

Product Specifications

Additional Formats

• Alexa Fluor 488
• APC
• eFluor 450
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

This 9E2 monoclonal antibody reacts with human CD335 (NKp46), a member of the natural cytotoxicity receptor family including NKp30 and NKp44. CD335 is expressed on NK cells and plays a role in activating and expanding resting NK cells through interactions with dendritic cells, contributing to the elimination of tumor and virus-infected cells. It also interacts with pancreatic beta cells during type 1 diabetes development. The 9E2 antibody has been reported to induce cytotoxicity.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Recommended Use:

• 5 µL (0.125 µg) per test
• Test defined as staining in 100 µL final volume
• Cell number: 10⁵–10⁸ cells/test

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample to antibody cocktails before combining with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample.

NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for optimized fluorescent signatures, minimizing spillover and spread.

Excitation: 509 nm
Emission: 614 nm
Laser: 488 nm (Blue)

Target Information

NKp46 (CD335, NCR1) is a cytotoxicity-activating receptor that enhances NK cell-mediated tumor lysis. It consists of two Ig-like domains assembled with leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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