Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (9E2), NovaFluor Red 685, eBioscience

Applications

• Tested Application: Flow Cytometry (Flow)
• Recommended Dilution: 5 µL (0.4 µg)/test

Product Specifications

Additional Available Formats

• Alexa Fluor 488
• APC
• eFluor 450
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Custom conjugation available

Product Specific Information

Description:
The 9E2 monoclonal antibody reacts with human CD335 (NKp46), a member of the natural cytotoxicity receptor family (NKp30, NKp44). CD335 is expressed on NK cells and functions as a type I transmembrane glycoprotein involved in activating and expanding resting NK cells through interaction with dendritic cells, contributing to the elimination of tumor and virus-infected cells. It also interacts with pancreatic beta cells during type 1 diabetes development. The 9E2 antibody has been reported to induce cytotoxicity.

Each product includes one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Pre-titrated and tested on normal human peripheral blood cells
• Recommended use: 5 µL (0.125 µg) per test (final volume 100 µL, 10⁵–10⁸ cells/test)

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, and DAPI.
• Use Invitrogen LIVE/DEAD Fixable Dead Cell Stains for viability analysis with NovaFluor dyes.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology, based on novel nucleic acid dye structures that enable engineered fluorescent signatures with minimal spectral spillover and spread.

Excitation: 637 nm
Emission: 685 nm
Laser: 633–640 nm (Red)

Target Information

NKp46 (CD335, NCR1) is a cytotoxicity-activating receptor expressed on NK cells, enhancing their ability to mediate tumor cell lysis. It contains two Ig-like domains related to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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