Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (9E2), NovaFluor Blue 610-70S, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.8 µg)/test

Product Specifications

Additional Formats Available

• Alexa Fluor 488
• APC
• eFluor 450
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Custom conjugation available upon request

Product Specific Information

Description:
The 9E2 monoclonal antibody recognizes human CD335 (NKp46), a member of the natural cytotoxicity receptor family expressed on NK cells. CD335 is a type I transmembrane glycoprotein involved in activating and expanding resting NK cells through interactions with dendritic cells, enabling elimination of tumor and virus-infected cells. It also interacts with pancreatic beta cells during the development of type 1 diabetes. The 9E2 antibody has been reported to induce cytotoxicity.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported:
Suitable for flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
Recommended use: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample to antibody cocktails before combining with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates are based on Phiton™ technology, utilizing novel nucleic acid dye structures engineered for optimized fluorescent signatures with reduced spillover and spread.

Excitation: 509 nm
Emission: 614 nm
Laser: 488 nm (Blue)

Target Information

NKp46 (CD335, NCR1) is a cytotoxicity-activating receptor that enhances the efficiency of activated NK cells in mediating tumor cell lysis. It consists of two Ig-like domains associated with leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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