Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (9E2), NovaFluor Blue 660-120S, eBioscience

Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (9E2), NovaFluor Blue 660-120S

Applications

• Flow Cytometry (Flow)

Tested Dilution

• 5 µL (0.8 µg)/test

Product Specifications

Additional Formats Available

• Alexa Fluor 488
• APC
• eFluor 450
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Red 685
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

This 9E2 monoclonal antibody reacts with human CD335 (NKp46), a natural cytotoxicity receptor expressed on NK cells. CD335 is a type I transmembrane glycoprotein that activates and expands resting NK cells upon interaction with dendritic cells, enabling elimination of tumor and virus-infected cells. It also interacts with pancreatic beta cells during type 1 diabetes development. The 9E2 antibody has been reported to induce cytotoxicity.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-titrated and validated for flow cytometry on normal human peripheral blood cells. Recommended usage is 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample to antibody cocktails.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, engineered for optimized fluorescent signatures minimizing spillover and spread.

Spectral Information:
Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

Target Information

NKp46 (CD335, NCR1) is a cytotoxicity-activating receptor that enhances NK cell-mediated tumor cell lysis. It comprises two Ig-like domains associated with leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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