Thermo Fisher Scientific Phospho-RPH3A (Ser234) Polyclonal Antibody
Rat RPH3A 단백질의 Ser234 인산화 부위를 인식하는 rabbit polyclonal antibody로, Western blot과 Immunocytochemistry에 적합합니다. Affinity chromatography로 정제되었으며, -20°C에서 보관합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:400 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 of rat RPH3A conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2181147 |
Product Specific Information
- This antibody is predicted to react with mouse based on 100% sequence homology.
- In Western blot, this antibody is specific for the ~82 kDa rabphilin protein phosphorylated at Ser234.
- Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
Target Information
The carboxy-terminal of rabphilin-3A consists of two C2 domains (A and B), and the amino-terminal (residues 45–170) contains a cysteine-rich region with two zinc finger motifs.
Rabphilin-3A belongs to the C-type tandem C2 protein family, which includes synaptotagmins and Doc2.
It is expressed in neuroendocrine cells and co-localizes with Rab3A on synaptic vesicles and chromaffin granules.
Rabphilin-3A binds Rab3a/GTP/Mg²⁺ within amino-terminal residues 45–170 and also binds calcium ions and phosphatidylinositol 4,5-bisphosphate-containing lipid vesicles within its C2 domains.
Rabphilin-3A acts as a positive regulator of calcium-dependent exocytosis, whereas Rab3a negatively regulates exocytosis.
Although associated, Rabphilin-3A and Rab3a influence exocytosis independently. Rabphilin-3A’s effects are likely mediated through interactions with an unknown factor recognizing the Rab3 binding domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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