Thermo Fisher Scientific T-bet Monoclonal Antibody (eBio4B10 (4B10)), eFluor 660, eBioscience
인간 및 생쥐 T-bet 단백질을 특이적으로 인식하는 eBio4B10 모노클론 항체로, eFluor 660 형광체가 결합되어 있습니다. Flow cytometry용으로 검증되었으며 Th1 세포 연구에 적합합니다. 651/668 nm 형광 특성, 0.2 mg/mL 농도, 4°C 보관.
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Thermo Fisher Scientific T-bet Monoclonal Antibody (eBio4B10 (4B10)), eFluor 660
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 3 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 67 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rhesus monkey |
| Published Species | Hamster, Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 660 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio4B10 (4B10) |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10597459 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 660
- APC
- eFluor 450
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-Cyanine5.5
- Custom conjugation available
Product Specific Information
The eBio4B10 monoclonal antibody reacts with mouse and human T-bet, a Th1-specific T-box transcription factor critical for Th1 CD4+ T cell lineage development.
T-Bet expression is induced by IFN-γ and regulates its own expression along with IL-12Rβ and IL-2, also contributing to class-switch recombination in B cells.
Applications Reported: Suitable for intracellular staining and flow cytometric analysis.
Applications Tested: Validated for intracellular staining and flow cytometry of human peripheral blood cells at ≤ 0.5 µg/test (100 µL final volume).
Optimal antibody titration is recommended per assay.
Use with Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00) and refer to BestProtocols® for intracellular staining (Protocol B).
Fluorophore Information:
eFluor® 660 replaces Alexa Fluor® 647.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
T-bet (TBX21) is a T-cell specific transcription factor containing a single T-box DNA-binding domain. It regulates expression of interferon-gamma and directs Th1 lineage development.
T-bet cooperates with Stat4 to program chromatin architecture for Th1 gene expression. Genetic variations are associated with aspirin-induced asthma and nasal polyps.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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