Thermo Fisher Scientific T-bet Monoclonal Antibody (eBio4B10 (4B10)), eFluor 660, eBioscience

Thermo Fisher Scientific T-bet Monoclonal Antibody (eBio4B10 (4B10)), eFluor 660

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• APC
• eFluor 450
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-Cyanine5.5
• Custom conjugation available

Product Specific Information

The eBio4B10 monoclonal antibody reacts with mouse and human T-bet, a Th1-specific T-box transcription factor critical for Th1 CD4+ T cell lineage development.
T-Bet expression is induced by IFN-γ and regulates its own expression along with IL-12Rβ and IL-2, also contributing to class-switch recombination in B cells.

Applications Reported: Suitable for intracellular staining and flow cytometric analysis.
Applications Tested: Validated for intracellular staining and flow cytometry of human peripheral blood cells at ≤ 0.5 µg/test (100 µL final volume).
Optimal antibody titration is recommended per assay.

Use with Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00) and refer to BestProtocols® for intracellular staining (Protocol B).

Fluorophore Information:
eFluor® 660 replaces Alexa Fluor® 647.

• Excitation: 633–647 nm
• Emission: 668 nm
• Laser: Red
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

T-bet (TBX21) is a T-cell specific transcription factor containing a single T-box DNA-binding domain. It regulates expression of interferon-gamma and directs Th1 lineage development.
T-bet cooperates with Stat4 to program chromatin architecture for Th1 gene expression. Genetic variations are associated with aspirin-induced asthma and nasal polyps.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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