Thermo Fisher Scientific Cardiac Myosin Heavy Chain Monoclonal Antibody (3-48)
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Cardiac Myosin Heavy Chain 단백질을 인식하는 Mouse monoclonal 항체로 다양한 종에 반응합니다. WB, IHC, ICC, ELISA 등에서 사용 가능하며, 높은 특이성과 친화도를 보입니다. 보존제 없이 PBS 용액 형태로 제공되며 연구용으로 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | View 2 publications |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | View 1 publication |
| ELISA | Assay-dependent | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Human, Mouse, Pig, Rabbit, Rat |
| Published Species | Human, Mouse, Pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3-48 |
| Immunogen | Purified, full-length, native human protein (Cardiac Myosin Heavy Chain, UniProt ID: A7E2Y1-1, antigen range: 1–1983) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | IgG fraction |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2147173 |
Product Specific Information
This antibody is specific for myosin alpha and beta heavy chains.
Affinity constants:
- Human ventricular myosin heavy chains: 3.33 × 10⁸ M⁻¹
- Human atrial myosin heavy chains: 1.48 × 10⁸ M⁻¹
- Human skeletal muscle myosin: 1.06 × 10⁸ M⁻¹
- Canine cardiac myosin heavy chains: 2.06 × 10⁸ M⁻¹
Cross-reactivity:
- Reacts with heavy chains of human myosin from slow skeletal muscle, fast skeletal muscle, eye muscle, and streptococcal M5 peptide.
- Does not react with human platelets, smooth muscle myosin heavy chains, myosin light chains, or myosin fragments in blood from myocardial infarction patients.
- No reactivity with other human tissue antigens or blood cells.
Recommended positive control: Slow skeletal muscle, fast skeletal muscle, eye muscle.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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