Thermo Fisher Scientific ZP2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 651-745 of human ZP2 (NP_0034511) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ZP2,uniProtId:Q05996-1,ncbiNodeId:9606,antigenRange:651-745,antigenLength:745,antigenImageFileName:PA5-87653_ZP2_Q05996-1_Rabbit.svg,antigenImageFileNamePDP:PA5-87653_ZP2_Q05996-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.87 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804322

Product Specific Information

Immunogen sequence: KMTVSLPGPI LLLSDDSSFR GVGSSDLKAS GSSGEKSRSE TGEEVGSRGA MDTKGHKTAG DVGSKAVAAV AAFAGVVATL GFIYYLYEKR TVSNH; Positive Samples: SH-SY5Y, HL-60, SW620, Mouse liver, Rat ovary; Cellular Location: Cell membrane, Single-pass type I membrane protein, extracellular matrix, extracellular space

Target Information

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.