Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), NovaFluor Blue 610-30S, eBioscience

Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), NovaFluor Blue 610-30S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–786)
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue series (510–800)
• NovaFluor Yellow series (570, 660)
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright series (436–780)
• Request custom conjugation available

Product Specific Information

The 61D3 monoclonal antibody reacts with human CD14, a 53–55 kDa GPI-linked glycoprotein expressed on monocytes, macrophages, and some dendritic cells. Complexes of LPS and LBP bind with high affinity to monocytes via CD14.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells. Recommended use at 5 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Compatibility Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01):

• Improved formulation over CellBlox.
• Required for optimal staining with NovaFluor conjugates.
• Recommended addition: 5 µL per sample or per antibody cocktail (100 µL cell sample).

NovaFluor Technology:

• Based on Phiton™ technology utilizing nucleic acid dye structures.
• Designed for engineered fluorescent signatures with minimal spillover and spread.

Excitation: 509 nm
Emission: 614 nm
Laser: 488 nm (Blue)

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein expressed on monocytes, macrophages, and neutrophils. It functions as a multifunctional lipopolysaccharide receptor and exists in both membrane-bound and soluble forms. CD14 interacts with LBP for LPS binding and plays roles in immune response, apoptosis, and inflammation. Dysregulation is associated with conditions such as Mycobacterium chelonae infection and Croup.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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