Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), NovaFluor Blue 585, eBioscience

Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), NovaFluor Blue 585, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 5 µL (0.6 µg)/test
• Publications: View 1 publication

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 421–786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue 510, 555, 610–800
• NovaFluor Yellow 570, 660
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436–780
• Request custom conjugation available

Product Specific Information

The 61D3 monoclonal antibody reacts with human CD14, a 53–55 kDa GPI-linked glycoprotein expressed on monocytes, macrophages, and some dendritic cells. CD14 binds LPS-LBP complexes with high affinity.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells. Recommended use: 5 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining. Add 5 µL per sample before mixing with cells.
• For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for optimized fluorescence signatures minimizing spillover and spread.

Excitation: 494 nm
Emission: 585 nm
Laser: 488 nm (Blue)

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein expressed on mature monocytes, macrophages, and neutrophils. It functions as a lipopolysaccharide receptor and exists in both membrane-bound and soluble forms. CD14 participates in immune responses to bacterial LPS, apoptotic cell binding, and inflammatory processes. Dysregulation is associated with infections (e.g., Mycobacterium chelonae) and respiratory conditions such as croup.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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