Thermo Fisher Scientific CDKN1B Polyclonal Antibody
다중 종 반응성을 가진 Rabbit Polyclonal CDKN1B (p27 Kip1) 항체로, WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 적합합니다. 세포 주기 조절 단백질 검출에 유용하며, Protein A 정제 및 고농도(1 mg/mL) 액상 형태로 제공됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:500 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human CDKN1B, amino acids 101–198 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to act as a mediator of TGFβ-induced G1 arrest. It is a key regulator of cell cycle progression and a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms complexes with cyclin D-CDK4 and contributes to the assembly, stability, and modulation of CCND1-CDK4 activation. Depending on its phosphorylation state and stoichiometry, p27 Kip1 can act as either an inhibitor or activator of cyclin D-CDK4 complexes. This protein (~27 kDa) undergoes CDK-dependent phosphorylation and ubiquitination by SCF complexes, which is essential for the transition from quiescence to the proliferative state.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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