Thermo Fisher Scientific CKMB Human ProcartaPlex Simplex Kit

Invitrogen™ CKMB Human ProcartaPlex™ Simplex Kit

The Human CKMB Simplex ProcartaPlex Kit measures CKMB protein and is designed to be combinable with other Simplex kits, allowing you to create your own multiplex panel utilizing Luminex xMAP technology for protein detection and quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is required per assay plate regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the sandwich ELISA principle, using two highly specific antibodies binding to different epitopes of one protein to quantitate multiple protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and provide analyzed results within four hours.

• More results per sample — measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology — highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures identifiable by Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the target protein. Each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are detected with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). This allows analysis of multiple targets in a single well.

The key difference between a ProcartaPlex assay and a traditional ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, allowing reagents to remain free-floating. The Luminex instrument uses two lasers—one to distinguish bead spectral signatures and another to quantify RPE fluorescence, proportional to the protein amount in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time as a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species: human, mouse, rat, nonhuman primate, porcine, and canine.

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