Thermo Fisher Scientific CD43 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:200
• Recommended positive control: Tonsil tissue

Product Specifications

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with the primary antibody for 30 minutes at room temperature.
A suggested positive control is tonsil tissue.

Target Information

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with a high negative charge, expressed on the surface of most hematopoietic cells.
It contributes to a repulsive barrier that interferes with cellular adhesion, but can also promote leukocyte aggregation.
Through interactions with actin-binding proteins ezrin and moesin, CD43 regulates T-cell morphology and cell-cell interactions during lymphocyte trafficking.
CD43 signaling enhances LFA-1 adhesiveness while counteracting LFA-1 induction via other receptors.
Expression of CD43 induces tumor suppressor p53 protein; however, in cases of p53 and ARF deficiency, CD43 promotes tumor proliferation and viability.
CD43 is an important modulator of leukocyte function.
Diseases associated with CD43 dysfunction include Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.