Thermo Fisher Scientific CD3 Monoclonal Antibody (SK7), PE-Cyanine5.5, eBioscience
인간 및 침팬지 CD3e 인식용 단클론 항체(SK7)로, PE-Cyanine5.5로 결합된 형광 표지 항체입니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 고순도 정제 및 안정적 신호를 제공합니다. 4°C 암소 보관, 동결 금지.
- 카탈로그번호
- 35-0036-4x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD3 Monoclonal Antibody (SK7), PE-Cyanine5.5, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 15 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chimpanzee, Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5.5, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SK7 |
| Conjugate | PE-Cyanine5.5 |
| Excitation/Emission Max | 564/692 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11218900 |
Additional Formats Available
APC, APC-eFluor 780, Brilliant UV 395, Brilliant UV 496, Brilliant UV 563, Brilliant UV 615, Brilliant UV 661, Brilliant Violet 650, eFluor 450, FITC, Functional Grade, NovaFluor Blue 510, NovaFluor Blue 610-70S, NovaFluor Blue 660-120S, NovaFluor Blue 690, NovaFluor Red 725, NovaFluor Red 755, NovaFluor Yellow 610, NovaFluor Yellow 690, PE, PE-Cyanine7, PerCP-Cyanine5.5, PerCP-eFluor 710, Super Bright 436, Super Bright 600, Super Bright 702, Super Bright 780.
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Product Specific Information
The SK7 monoclonal antibody reacts with human and chimpanzee CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain, along with CD3 gamma and delta, is essential for proper assembly and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. SK7 and UCHT1 recognize overlapping epitopes, while OKT3 and SK7 recognize distinct ones. The SK7 antibody also recognizes chimpanzee CD3.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested for flow cytometry using normal human peripheral blood cells. Recommended usage is 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Light Sensitivity: Protect from light; PE-Cyanine5.5 is sensitive to photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark.
Excitation: 488–561 nm
Emission: 695 nm
Compatible Lasers: Blue, Green, Yellow-Green
Target Information
The CD3 subunit complex is critical for transducing antigen-recognition signals into T cells and regulating TCR surface expression. It includes CD3 gamma, delta, epsilon, and zeta subunits, encoded on chromosome 11. These subunits associate with intracellular signaling molecules via tyrosine-based motifs. CD3 is expressed on 68–82% of normal peripheral blood lymphocytes and 65–85% of thymocytes, but not on B or NK cells. CD3 gene defects are associated with immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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