Thermo Fisher Scientific RAB9 Monoclonal Antibody (Mab9)
다양한 종에서 Rab9 단백질을 인식하는 마우스 단클론 항체. Western blot, IHC, ICC, IP 등 다양한 응용에 적합. 비접합형 액상 항체로, 고순도 Protein A 정제. Rab9 단백질의 세포 내 수송 및 재활용 연구에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 | View 5 publications |
| Immunohistochemistry (IHC) | – | View 5 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20 | – |
| Immunocytochemistry (ICC/IF) | 1:200–1:500 | View 6 publications |
| Flow Cytometry (Flow) | – | View 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | – |
| Immunomicroscopy (IM) | Assay-dependent | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Hamster, Human, Non-human primate, Rat |
| Published Species | Hamster, Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Mab9 |
| Immunogen | Recombinant canine rab 9a |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2175599 |
Product Specific Information
MA3-067 recognizes both prenylated and non-prenylated Rab9 from canine, human, monkey, rat, hamster, and bovine tissues. This antibody does not cross-react with other Rab family members.
It has been successfully used in Western blot, immunofluorescence, EM immunocytochemistry, and immunoprecipitation. In Western blot analysis, it detects an approximately 26 kDa Rab9 protein from K562 cell extract.
The immunogen for MA3-067 is recombinant canine Rab9a.
Target Information
Rab proteins are Ras-like GTPases involved in intracellular protein transport. Members of the Rab family mediate docking and fusion of transport vesicles between cellular compartments.
Rab9 is essential for trafficking mannose 6-phosphate receptor between the late endosome and the trans-Golgi network (TGN). By facilitating receptor transport, Rab9 enables efficient recycling of cellular trafficking components.
Rab9 requires prenylation by two 20-carbon geranylgeranyl groups at its C-terminus for function. Most prenylated Rab9 is membrane-bound, but 10–20% exists in the cytosol bound to GDP dissociation inhibitor-alpha (GDI-alpha), which recycles Rab9 between membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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