Thermo Fisher Scientific RAB9 Monoclonal Antibody (Mab9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

-

Immunocytochemistry (ICC/IF)

1:200-1:500

View 6 publications 6 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

-

Immunomicroscopy (IM)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Dog, Hamster, Human, Non-human primate, Rat

Published species

Hamster, Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

Mab9

Immunogen

Recombinant canine rab 9a.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2175599

Product Specific Information

MA3-067 recognizes prenylated and non-prenylated rab 9 from canine, human, monkey, rat, hamster and bovine tissues. This antibody does not cross-react with other rab family members.

MA3-067 has been successfully used in Western blot, immunofluorescence, EM immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~26 kDa protein representing rab 9 from K562 cell extract.

The MA3-067 antigen is recombinant canine rab 9a.

Target Information

Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.