Thermo Fisher Scientific Mouse anti-Canine IgE Secondary Antibody

Applications

Tested Dilution

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Dog

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Secondary Antibody

Clone

E6-2A1

Immunogen

Affinity purified IgE preparation from heavily parasitized and allergic dog serum.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

<0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538146

Target

IgE

Antibody Form

Whole Antibody

Product Specific Information

This antibody recognizes canine IgE, and does not cross react with canine IgM, IgA, IgG1 or IgG2. Western blot analysis against affinity purified IgE shows a single major band of 62-65kDa under reducing conditions.

Target Information

Anti-Canine secondary antibodies are affinity-purified antibodies with well-characterized specificity for canine (dog) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.