
Thermo Fisher Scientific Mouse anti-Canine IgE Secondary Antibody
개 IgE를 특이적으로 인식하는 Mouse monoclonal 2차 항체로, Western blot 및 ELISA에 사용 가능. 비결합형(unconjugated) 형태로 높은 특이성과 민감도를 제공. Protein A로 정제되어 신뢰성 높은 결과를 지원. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Secondary Antibody |
| Clone | E6-2A1 |
| Immunogen | Affinity purified IgE preparation from heavily parasitized and allergic dog serum |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | <0.1% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538146 |
| Target | IgE |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody recognizes canine IgE and does not cross-react with canine IgM, IgA, IgG1, or IgG2.
Western blot analysis against affinity purified IgE shows a single major band of 62–65 kDa under reducing conditions.
Target Information
Anti-Canine secondary antibodies are affinity-purified antibodies with well-characterized specificity for canine (dog) immunoglobulins.
They are useful in the detection, sorting, or purification of specific targets.
Secondary antibodies enable the use of various detection systems (e.g., HRP, AP, fluorescence) and provide enhanced sensitivity through signal amplification.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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