Thermo Fisher Scientific Phospho-IRS1 (Ser323) Polyclonal Antibody

Applications

Product Specifications

Target Information

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1), and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine, and tyrosine residues in various tissues. Casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser99 is an additional site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser270, Ser374, and Ser641), and the motif around Ser270 is located in the phosphotyrosine binding domain of IRS-1, which is responsible for interaction with the insulin receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.