Thermo Fisher Scientific HES5 Recombinant Rabbit Monoclonal Antibody (ARC1740)
HES5 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ELISA에 적합합니다. 인간, 생쥐, 랫드 시료에 반응하며, HEK293 세포에서 발현되었습니다. 고순도의 친화 크로마토그래피 정제 및 안정한 PBS 버퍼에 보관됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1740 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 12–142 of human HES5 (Q5TA89) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.9 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898131 |
Product Specific Information
- Positive Samples: Mouse brain, Mouse spleen, Rat brain, Rat spleen
- Immunogen Sequence:
SPKEKNRLRK PVVEKMRRDR INSSIEQLKL LLEQEFARHQ PNSKLEKADI LEMAVSYLKH SKAFVAAAGP KSLHQDYSEG YSWCLQEAVQ FLTLHAASDT QMKLLYHFQR PPAAPAAPAK EPKAPGAAPP P
Target Information
Hairy and Enhancer-of-split homologues 5 (HES5) is a helix-loop-helix transcriptional factor that negatively regulates cell differentiation during embryogenesis. It contains one basic helix-loop-helix (bHLH) domain and one Orange domain. HES5 is reported to be a downstream effector of the Notch signaling pathway and is expressed in advanced ovarian serous adenocarcinomas as well as in cervical squamous carcinoma. Reports also suggest that HES5 is abundantly expressed in osteoarthritis along with Notch1 and Jagged1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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