Thermo Fisher Scientific USP9X Recombinant Rabbit Monoclonal Antibody (ARC1748)
USP9X 단백질을 인식하는 토끼 유래 재조합 단클론 항체로, Western blot, IHC, ICC/IF, ELISA에 적합합니다. 인간, 마우스, 랫트 반응성이 있으며, HEK293 세포에서 발현되었습니다. 고순도 친화 크로마토그래피 정제 및 안정한 액상 형태로 제공됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1748 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1–100 of human USP9X (Q93008) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.37 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898134 |
Product Specific Information
Positive Samples: 293T, PC-3, C6, U-251MG, Mouse testis, Mouse brain
Immunogen Sequence:
MTATTRGSPV GGNDNQGQAP DGQSQPPLQQ NQTSSPDSSN ENSPATPPDE QGQGDAPPQL EDEEPAFPHT DLAKLDDMIN RPRWVVPVLP KGELEVLLEA
Target Information
USP9X is an ubiquitin-specific protease involved in the removal and recycling of ubiquitin from proteins degraded in proteasomes. As a deubiquitinating enzyme, USP9X has a critical function in tight junction biogenesis by controlling the levels of the exchange factor for Arf6 (EFA6), a protein shown to facilitate tight junction formation during a narrow temporal window preceding the establishment of cell polarity. USP9X is also located on the X-chromosome but escapes X-inactivation, and defects in USP9X may cause gonadal degeneration in Turner syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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