Thermo Fisher Scientific Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody (ARC1745)
Cytokeratin 1 단백질을 인식하는 재조합 토끼 단클론 항체로, WB, IHC, ICC, ELISA 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 시료에 반응하며, HEK293 세포에서 발현되었습니다. 고순도 친화 크로마토그래피 정제 및 안정한 PBS 기반 저장 버퍼를 사용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:200–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1745 |
| Immunogen | Synthetic peptide corresponding to amino acids 545–644 of human Cytokeratin 1 (KRT1, P04264) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898133 |
Product Specific Information
- Positive Samples: Mouse brain, Rat brain, A375
- Immunogen Sequence:
GGGGRGSYGS GGSSYGSGGG SYGSGGGGGG HGSYGSGSSS GGYRGGSGGG GGGSSGGRGS
GGGSSGGSIG GRGSSSGGVK SSGGSSSVKF VSTTYSGVTR
Target Information
Cytokeratins are a subfamily of intermediate filament proteins characterized by remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between approximately 40 kDa and 68 kDa and have an isoelectric pH between 4.9 and 7.8.
Each epithelial tissue expresses specific cytokeratin subtypes that reflect its type and differentiation state. Cytokeratin subtype expression patterns are valuable in distinguishing different epithelial malignancies. Cytokeratin antibodies assist in differential tumor diagnosis using immunohistochemistry and are also useful in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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