Thermo Fisher Scientific Phospho-ZAP70 (Tyr493) Polyclonal Antibody
인간 ZAP70 단백질의 Tyr493 인산화 부위를 인식하는 Rabbit Polyclonal 항체입니다. Western blot 및 immunoprecipitation에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 제공되며 4°C에서 보관합니다.
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Applications
Western Blot (WB)
- Assay-dependent
Immunoprecipitation (IP)
- 2–5 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region including tyrosine 493 (phosphorylated) of Human zeta-chain (TCR) associated protein kinase 70kDa |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed exclusively in T-cells and natural killer cells and is essential for T-cell receptor activation. Upon TCR engagement, ZAP70 is phosphorylated on tyrosines 292, 315, and 319 in the interdomain B region between the SH2 and kinase domains.
Phosphorylation of tyrosines 315 (Vav binding site) and 319 (Lck binding site) enhances ZAP70 function in lymphocyte signaling, while phosphorylation of tyrosine 292 attenuates signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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