Thermo Fisher Scientific Phospho-ZAP70 (Tyr493, Tyr494) Polyclonal Antibody
인산화된 ZAP70(Tyr493, Tyr494)을 인식하는 Rabbit Polyclonal Antibody. Western blot 및 Immunoprecipitation에 적합. 항원 친화 크로마토그래피로 정제된 액상 제품. 인간에 반응하며 마우스에도 예측 반응. 연구용 전용.
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Applications
Western Blot (WB)
- Assay-dependent
Immunoprecipitation (IP)
- 2–5 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region including tyrosines 493 and 494 (when phosphorylated) of Human zeta-chain (TCR) associated protein kinase 70kDa |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed exclusively in T-cells and natural killer cells and is essential for T-cell receptor (TCR) activation. Upon TCR engagement, ZAP70 becomes phosphorylated on tyrosines 292, 315, and 319 in the interdomain B region between the SH2 and kinase domains. Phosphorylation at tyrosines 315 (Vav binding site) and 319 (Lck binding site) enhances signaling, while phosphorylation at tyrosine 292 attenuates activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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