Thermo Fisher Scientific PARP1 Recombinant Rabbit Monoclonal Antibody (2G8N7)
PARP1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 적합합니다. HEK293 세포에서 발현되었으며, Human, Mouse, Rat에 반응합니다. 고순도의 액상 형태로 제공되며, -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2G8N7 |
| Immunogen | Synthetic peptide corresponding to amino acids 700–800 of human PARP1 (UniProt ID: P09874) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS (pH 7.3) with 0.05% BSA, 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3094181 |
Product Specific Information
Immunogen sequence:
KLSKRQIQAA YSILSEVQQA VSQGSSDSQI LDLSNRFYTL IPHDFGMKKP PLLNNADSVQ AKVEMLDNLL DIEVAYSLLR GGSDDSSKDP IDVNYEKLKT D
Target Information
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form, NAD) as a substrate to catalyze the transfer of ADP-ribose to various nuclear protein acceptors. Proteolysis of PARP to its stable 85 kDa fragment is an early marker of apoptosis and is mediated by the caspase CPP32 protein. Cleavage occurs between Asp216 and Gly217, a conserved site across species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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