Thermo Fisher Scientific LAG3 Monoclonal Antibody (OTI8F6), TrueMAB
Human LAG3 단백질을 표적으로 하는 Mouse IgG2b 모노클로날 항체로, Western blot, IHC, Flow cytometry 등에 사용 가능. 고순도 Affinity chromatography 정제, Lyophilized 형태로 제공되며 연구용으로 적합.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:2,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 | |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI8F6 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 66–332 of human LAG3 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
LAG-3 (CD223) is a 70-kDa surface glycoprotein of the Ig superfamily, homologous to CD4. It binds to MHC class II with higher affinity than CD4 and negatively regulates T cell activation and proliferation.
LAG-3 is expressed on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells generally express higher levels of LAG-3 than CD4+ T cells. Coexpression of LAG-3 and CD49b identifies Type 1 regulatory T cells (Tr1 cells) in humans and mice.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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