Thermo Fisher Scientific GAD1 Monoclonal Antibody (OTI5D8), TrueMAB
GAD1 단백질을 인식하는 Mouse monoclonal antibody (clone OTI5D8). Western blot, IHC, IP에 적합하며, 1 mg/mL 농도의 동결건조 형태로 제공. 인간 GAD1에 반응하며, PBS buffer(8% trehalose) 내 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunoprecipitation (IP) | 4 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5D8 |
| Immunogen | Full-length protein expressed in 293T cell transfected with human GAD1 expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
GAD-65 and GAD-67 are members of the group II decarboxylase family that catalyze the conversion of L-glutamic acid to GABA (gamma-aminobutyric acid).
- GAD-65: Localized to synaptic vesicle membranes, activated during increased neurotransmission demand.
- GAD-67: Distributed throughout the cell, responsible for basal GABA synthesis.
Loss of GAD-65 impairs GABA neurotransmission, while loss of GAD-67 is lethal.
Two isoforms exist for GAD-67 due to alternative splicing: the predominant GAD-67 and the minor GAD-25.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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