Thermo Fisher Scientific CD101 Monoclonal Antibody (Moushi101), PE, eBioscience

Thermo Fisher Scientific CD101 Monoclonal Antibody (Moushi101), PE, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test
Publications: View 7 publications

Product Specifications

Additional Available Formats

• Alexa Fluor 700
• APC
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Yellow 730
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The monoclonal antibody Moushi101 recognizes mouse CD101, also known as IGSF2 and V7 (7 IgV domains). CD101 is a transmembrane glycoprotein containing seven Ig-like domains and a short cytoplasmic tail with several phosphorylation sites. Cross-linking CD101 can antagonize TCR/CD3-mediated lymphocyte activation.
CD101 is expressed on monocytes, granulocytes, dendritic cells, Langerhans cells, and activated T cells. It is also found on a subset of CD25⁺Foxp3⁺ T regulatory cells with higher suppressive properties in vitro and in vivo.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.25 µg/test. A test is defined as the amount of antibody to stain cells in 100 µL final volume. Cell number can range from 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal performance.
Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD101 (V7 antigen) is a member of the immunoglobulin superfamily and forms a disulfide-linked homodimer. It is expressed on monocytes, granulocytes, dendritic cells, and T lymphocytes, playing a role in cell activation.
CD101⁺CD28⁺ cells respond strongly to CD28 signaling, and anti-CD101 can enhance proliferation when combined with anti-CD28 or suboptimal anti-CD3.
Mouse CD101 is expressed on a subpopulation of regulatory T cells (CD4⁺, CD25⁺, Foxp3⁺) with high suppressor activity. In human PBMCs, approximately 30% of Foxp3⁺ cells express CD101, though enhanced suppressive activity has not been confirmed.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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