Thermo Fisher Scientific CD98 Monoclonal Antibody (RL388), PE, eBioscience
Mouse CD98 단백질을 인식하는 RL388 클론의 단일클론 항체로 PE 형광표지되어 있습니다. Flow cytometry에 최적화된 시약이며 세포 활성화 및 칼슘 플럭스 연구에 적합합니다. 0.2 mg/mL 농도로 PBS 완충액에 보존되며 4°C 암소 보관 권장.
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Thermo Fisher Scientific CD98 Monoclonal Antibody (RL388), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 10 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RL388 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465792 |
Product Specific Information
The RL388 monoclonal antibody reacts with mouse CD98 (4F2 antigen, Ly-10). RL388 detects a disulfide-linked heterodimer complex (130 kDa) composed of a glycosylated heavy (86 kDa) and a nonglycosylated light (39 kDa) subunit. CD98 is expressed on activated lymphocytes and is involved in cell activation and calcium flux events.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on activated and resting mouse splenocytes. Recommended ≤0.5 µg per test (100 µL final volume). Optimal titration required.
Fluorescence Characteristics:
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD98 (4F2) is a type II transmembrane glycoprotein serving as the heavy chain of heterodimeric amino acid transporters (HATs). It interacts with beta1 integrins, enhancing ligand affinity and contributing to amino acid transport, cell fusion, activation, differentiation, growth, and apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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