Thermo Fisher Scientific Phospho-RSK Pan (Ser221, Ser227, Ser218, Ser232) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human RSK1/2/3/4 around the phosphorylation site of Ser221/227/s218/232. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RSK Pan,uniProtId:Q15349-1,ncbiNodeId:9606,antigenRange:218,antigenLength:733,antigenImageFileName:PA5-114637_RSK_Pan_Q15349-1_Rabbit.svg,antigenImageFileNamePDP:PA5-114637_RSK_Pan_Q15349-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:RSK Pan,uniProtId:Q9UK32-1,ncbiNodeId:9606,antigenRange:232,antigenLength:745,antigenImageFileName:PA5-114637_RSK_Pan_Q9UK32-1_Rabbit.svg,antigenImageFileNamePDP:PA5-114637_RSK_Pan_Q9UK32-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:RSK Pan,uniProtId:Q15418-1,ncbiNodeId:9606,antigenRange:221,antigenLength:735,antigenImageFileName:PA5-114637_RSK_Pan_Q15418-1_Rabbit.svg,antigenImageFileNamePDP:PA5-114637_RSK_Pan_Q15418-1_Rabbit_PDP.jpeg,sortOrder:3},{targetFamily:RSK Pan,uniProtId:P51812-1,ncbiNodeId:9606,antigenRange:227,antigenLength:740,antigenImageFileName:PA5-114637_RSK_Pan_P51812-1_Rabbit.svg,antigenImageFileNamePDP:PA5-114637_RSK_Pan_P51812-1_Rabbit_PDP.jpeg,sortOrder:4}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2899273

Product Specific Information

Antibody detects endogenous levels of RSK1/2/3/4 only when phosphorylated at Ser221/227/s218/232.

Target Information

The p90 ribosomal S6 kinases (RSKs) comprise a family of serine/threonine kinases that lie at the terminus of the ERK pathway. In humans, the RSK family consists of four isoforms (RSK1 to-4). RSK family members are unusual among serine/threonine kinases in that they contain two distinct kinase domains, both of which are catalytically functional. Theses kinase domains are activated in a sequential manner by a series of phosphorylations. RSK regulates gene expression via association and phosphorylation of transcriptional regulators including c-Fos, estrogen receptor, NFkappaB/IkappaB alpha, cAMP-response element-binding protein (CREB). ERK activates the C-terminal kinase of RSK, leading to activation of the N-terminal kinase. Members of the RSK family are present in the cytoplasm as well as the nucleus. Addition of growth factor to the cells results in the activation of both cytosolic and nuclear RSK and the translocation of the cytosolic RSK into the nucleus upon activation. The activation and nuclear translocation of RSK result in phosphorylation and activation of transcription factors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.