Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), eFluor 570, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

Unconjugated, Alexa Fluor 532, Alexa Fluor 561, Alexa Fluor 647, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV series (395–805), Brilliant Violet series (421–786), eFluor 450/506/615, FITC, NovaFluor Blue/Yellow series, Pacific Orange, PE, PE-Cyanine series, PerCP-Cyanine series, Super Bright series.
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Product Specific Information

The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, subpopulations of mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and plays a critical role in T-cell development and function. In T cells, CD4 associates with the protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.

Applications Reported:
Immunohistochemical staining of frozen tissue sections, microscopy, and immunocytochemistry.

Applications Tested:
Tested by immunohistochemistry of frozen mouse tissue; effective at ≤5 µg/mL. Titrate for optimal performance.

Filter Recommendation:
Use a filter that captures 570 nm emission (e.g., Excitation 545/25, 565LP, Emission 605/70). Standard Alexa Fluor® 555 or TRITC filters are acceptable.

Excitation / Emission: 555 nm / 570 nm

Target Information

CD4 is involved in recognition of MHC class II molecules and acts as a co-receptor for HIV. It is mainly expressed in T helper cells, as well as monocytes, macrophages, and dendritic cells. CD4 expression is found in thymus, lymph nodes, tonsils, spleen, and also in specific brain and gut regions. CD4 contributes to early T-cell activation via association with the TCR complex and tyrosine kinase Lck, and may mediate neuronal damage in immune-related CNS diseases.
(RefSeq, July 2017)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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