Thermo Fisher Scientific LAP (Latency Associated peptide) Monoclonal Antibody (FNLAP), PE-Cyanine7, eBioscience
Human LAP(TGFβ1) 검출용 FNLAP 단클론 항체로, Flow Cytometry에 최적화된 PE-Cyanine7 형광 표지 제품입니다. 5 µL/test로 사용하며, 4°C 암소 보관이 필요합니다. Blue/Green/Yellow-Green Laser에서 488–561 nm 여기, 775 nm 방출 특성을 가집니다.
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Applications
Tested Application
- Flow Cytometry (Flow)
Tested Dilution
- 5 µL (0.25 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FNLAP |
| Immunogen | E. coli derived hLAP-TGFβ, mixed with VB3A9 |
| Conjugate | PE-Cyanine7 |
| Excitation/Emission Max | 569/780 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Storage Conditions | 4°C, store in the dark, Do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_11063989 |
Additional Formats
Product Specific Information
Description:
The FNLAP monoclonal antibody reacts with human latency associated peptide (LAP, pro-TGF beta 1, LAP/TGF beta 1). TGF beta is synthesized as a precursor containing LAP at the N-terminus and mature TGF beta at the C-terminus. Cleavage between amino acids 278 and 279 forms LAP and TGF beta dimers, which associate non-covalently to form the small latent TGF beta complex. LAP is secreted into the extracellular matrix and also expressed on platelets and activated regulatory T cells, likely via GARP (LRRC32) binding.
Applications Reported:
- Flow cytometric analysis.
Applications Tested:
- Pre-titrated and validated for flow cytometric analysis of stimulated human peripheral blood cells.
- Recommended use: 5 µL (0.25 µg) per test (100 µL final volume).
- Cell count range: 10⁵–10⁸ cells/test (empirically determined).
Light Sensitivity:
- Tandem dye is sensitive to photo-induced oxidation. Protect from light.
Fixation:
- Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark.
- Minimal impact on brightness and FRET efficiency/compensation.
- Clone-specific performance should be confirmed empirically.
Optical Properties:
- Excitation: 488–561 nm
- Emission: 775 nm
- Compatible Lasers: Blue, Green, Yellow-Green
Filtration:
- 0.2 µm post-manufacturing filtered.
Target Information
TGF beta-1 is a cytokine of the transforming growth factor beta superfamily, found ubiquitously in tissues. It is stored in the extracellular matrix as a latent complex with its prodomain (LAP). Activation requires αV integrin binding to an RGD sequence in the prodomain, enabling release from the extracellular matrix via latent TGF-beta binding proteins (LTBP). The latent complex includes TGF-beta, LAP, and LTBP.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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